Transformedfromhumannon-smallcelllungcarcinomacellline, NCI-H1299 adhesivecell,usinglentivirusexpressing fireflyluciferase3gene.The Luciferase wasexpressedundertheenhancedEF1a promoter. ThePuromycinMarkerwasexpressedunderRsvpromoter.Thecelllinedemonstrateshigh levelofbioluminescence signalviafireflyluciferaseassay.Thefollowingexpresscassettewasintegratedintocellline’sgenome. 

EF-luc-Puro

Pleaseseedetails in ProductManual.

Soldat: 1vialx(2 x106cells)/vial 

Cat#SC053-L