ProductDescription:

  • Endotoxin-free
  • Animal-derivedproductfree
  • AvailableinBulk
  • LyophilizedandCarrierFree(CF)

RecombinanthumanFGFbasic-TS™isexpressedinhuman293cells.FGFbasicisaheparin-bindinggrowthfactorandcriticalforhumanembryonicstemcellstoremaininanundifferentiatedstateduringcellculture.FGFbasic-TS™isaproteinengineeredFGFbasictoenchanceitsThermo-stABIlitywithoutmodificationofBIOLOGicalfunction.FGFbasic-TS™consistsof154aminoacidsasa17kDa,non-glycosylatedmonomer.

TechnicalSpecifications:

  • Species:Human
  • Expression:HEK293CellExpressed
  • Activity:Typically≤0.5ng/mLEC50
  • Purity:>95%
  • Endotoxin:<1EU/µg
  • MolecularMass:17kDa,monomer,non-glycosylated
  • Formulation:1xPBS

PurityConfirmation:

TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.

ActivityAssay:

Theactivitywasdeterminedbythedose-dependentstimulationoftheproliferationoftheBalb/c3T3cellline.

ReconstitutionBuffer:

Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteininsterile1xPBScontaining0.1%endotoxin-freerecombinanthumanserumalbumin(HSA).